PB-TRE3Gbi-MCS-mScarlet3
(Plasmid
#259539)
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Purpose(Empty Backbone) Empty all-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression via a bi-directional TRE3G promoter. Includes an mScarlet3 reporter and an MCS for subcloning genes of interest.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUCM
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TRE3Gbi-MCS-mScarlet3 was a gift from Alexander Chesler (Addgene plasmid # 259539 ; http://n2t.net/addgene:259539 ; RRID:Addgene_259539) -
For your References section:
An inherited hypomorphic variant in PIEZO2 reveals structural features of mechanotransduction. Nickolls AR, Mulhall EM, Orlin DJ, Yarishkin O, Romero LO, Bell B, Hu X, Madden CA, Hardy RR, O'Brien GS, Donkervoort S, Saade DN, Lehky TJ, Foley AR, Bharucha-Goebel DX, Dastgir J, Vasquez V, Cordero-Morales JF, Bonnemann CG, Patapoutian A, Falgairolle M, Chesler AT. Neuron. 2026 Aug 24:S0896-6273(26)00585-4. doi: 10.1016/j.neuron.2026.07.030. 10.1016/j.neuron.2026.07.030 PubMed 42636812
