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PB-TRE3Gbi-hPIEZO1_N2205K-mScarlet3
(Plasmid #259541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259541 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUCM
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hPIEZO1_N2205K
  • Alt name
    PIEZO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7563
  • Mutation
    N2205K
  • Entrez Gene
    PIEZO1 (a.k.a. DHS, ER, FAM38A, LMPH3, LMPHM6, Mib)
  • Promoter pTRE3G-bidirectional

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Valeria Vasquez, UTHealth Houston

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This hPIEZO1 insert has some missense variants and one in-frame codon deletion not reported in wildtype reference sequences (e.g., UniProt Q92508) that do not appear to affect channel function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE3Gbi-hPIEZO1_N2205K-mScarlet3 was a gift from Alexander Chesler (Addgene plasmid # 259541 ; http://n2t.net/addgene:259541 ; RRID:Addgene_259541)

  • For your References section:

    An inherited hypomorphic variant in PIEZO2 reveals structural features of mechanotransduction. Nickolls AR, Mulhall EM, Orlin DJ, Yarishkin O, Romero LO, Bell B, Hu X, Madden CA, Hardy RR, O'Brien GS, Donkervoort S, Saade DN, Lehky TJ, Foley AR, Bharucha-Goebel DX, Dastgir J, Vasquez V, Cordero-Morales JF, Bonnemann CG, Patapoutian A, Falgairolle M, Chesler AT. Neuron. 2026 Aug 24:S0896-6273(26)00585-4. doi: 10.1016/j.neuron.2026.07.030. 10.1016/j.neuron.2026.07.030 PubMed 42636812
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