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Rad50_P2A-HA-BSD_N-Donor
(Plasmid #259554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259554 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Gateway
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD50
  • Alt name
    BSD-P2A-HA-FKBPv
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAD50 (a.k.a. NBSLD, RAD502, hRad50)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

use this Donor with PX330_sgRNA_rad50 make N-terminal dTAG Knockin cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rad50_P2A-HA-BSD_N-Donor was a gift from Junjie Chen (Addgene plasmid # 259554 ; http://n2t.net/addgene:259554 ; RRID:Addgene_259554)

  • For your References section:

    CRISPR Screening Identifies SMARCAL1 and MRN as Modulators of WRN Dependency in MSI-H Colorectal Cancer. Ma T, Wu J, Li S, Chen J. Cancer Res. 2026 Jul 22. doi: 10.1158/0008-5472.CAN-26-1023. 10.1158/0008-5472.CAN-26-1023 PubMed 42484294
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