pLH272
(Plasmid
#259580)
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PurposeRMCE exchange vector containing most of Aicda intron 1 and coding sequences (R190X)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLH28
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAicda intron 1
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SpeciesM. musculus (mouse)
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MutationR190X
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Entrez GeneAicda (a.k.a. Aid, Arp2)
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Tag
/ Fusion Protein
- HaloTag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLH272 was a gift from Kefei Yu (Addgene plasmid # 259580 ; http://n2t.net/addgene:259580 ; RRID:Addgene_259580) -
For your References section:
A dynamic RNA hub facilitates activation-induced cytidine deaminase recruitment to the immunoglobulin heavy-chain locus. Mikhova M, Kapanka M, Han L, Ungor G, Schmidt JC, Yu K. Mol Cell. 2026 Jun 23:S1097-2765(26)00383-7. doi: 10.1016/j.molcel.2026.06.012. 10.1016/j.molcel.2026.06.012 PubMed 42335900