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pLH260R174S
(Plasmid #259584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259584 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLH28
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aicda intron 1
  • Species
    M. musculus (mouse)
  • Mutation
    R174S
  • Entrez Gene
    Aicda (a.k.a. Aid, Arp2)
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLH260R174S was a gift from Kefei Yu (Addgene plasmid # 259584 ; http://n2t.net/addgene:259584 ; RRID:Addgene_259584)

  • For your References section:

    A dynamic RNA hub facilitates activation-induced cytidine deaminase recruitment to the immunoglobulin heavy-chain locus. Mikhova M, Kapanka M, Han L, Ungor G, Schmidt JC, Yu K. Mol Cell. 2026 Jun 23:S1097-2765(26)00383-7. doi: 10.1016/j.molcel.2026.06.012. 10.1016/j.molcel.2026.06.012 PubMed 42335900
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