Sox2-EGFP knockin donor template
(Plasmid
#259653)
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PurposeHomologous recombination donor template to generate Sox2-EGFP knockin mouse embryonic stem cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259653 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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SpeciesH. sapiens (human)
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sox2-EGFP knockin donor template was a gift from Charles Roberts (Addgene plasmid # 259653 ; http://n2t.net/addgene:259653 ; RRID:Addgene_259653) -
For your References section:
Mitotic bookmarking by SWI/SNF subunits. Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF, Green DR, Zhang J, Roberts CWM. Nature. 2023 Jun;618(7963):180-187. doi: 10.1038/s41586-023-06085-6. Epub 2023 May 24. 10.1038/s41586-023-06085-6 PubMed 37225980