pCDNA3 10 mut B-Myb
(Plasmid #25966)

• Depositing Lab: Rob Lewis
• Publication: Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-9.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B-Myb
• Species: M. musculus (mouse)
• Insert Size (bp): 2650
• Mutation: changed threonines 267, 408, 497, 519, 522 and 524 to valines, and serines 283, 396, 455, and 581 to alanines
• GenBank ID: X70472
• Entrez Gene: Mybl2 (a.k.a. B-Myb, Bmyb)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R.J.Watson Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, UK

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry