pAAV-hSyn1-EGFP-Sensor (tdT)-tTA2-WPRE
(Plasmid
#259669)
-
PurposeDetecting tdTomato mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct.
-
Depositing Lab
-
Depositing OrganizationRice University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepAAV-hSyn-Gluc-RMA-IRES-EGFP
-
Backbone manufacturerJerzy Szablowski, Addgene plasmid #189629
- Total vector size (bp) 6357
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRNA sensor for tdTomato
-
SpeciesSynthetic
- Promoter hSyn1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGGCATGGACGAGCTGTACA
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe sensor structure and components are based on the previous research and the uploaded plasmid (#192063).
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-hSyn1-EGFP-Sensor (tdT)-tTA2-WPRE was a gift from Jerzy Szablowski (Addgene plasmid # 259669 ; http://n2t.net/addgene:259669 ; RRID:Addgene_259669) -
For your References section:
Monitoring in vivo transcription with synthetic serum markers. Watanabe S, Lee S, Harb M, Nouraein S, Raisley E, Li H, Buitrago N, Pforr B, Szablowski JO. Nat Commun. 2026 May 27. doi: 10.1038/s41467-026-73486-2. 10.1038/s41467-026-73486-2 PubMed 42203776