Monitoring in vivo transcription with synthetic serum markers.
Watanabe S, Lee S, Harb M, Nouraein S, Raisley E, Li H, Buitrago N, Pforr B, Szablowski JO
Nat Commun. 2026 May 27. doi: 10.1038/s41467-026-73486-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 259647 | pAAV-TRE3g-Gluc-RMA | Expresses Gluc-RMA under the TRE3g promoter. |
| 259648 | pAAV-TRE3g-Nluc-RMA | Expresses nanoluc-RMA under the TRE3g promoter. |
| 259667 | CAG-EGP-Sensor (tdT)-tTA2-WPRE | The system detects tdTomato mRNA and induces tTA2 expression. tTA2 subsequently activates reporter gene expression from a TRE-driven construct. |
| 259668 | CAG-ADAR2-Sensor (tdT)-Gluc-RMA | Detecting tdTomato mRNA by synthetic serum marker Gluc-RMA. RMA production is accerelated by ADAR2 overexpression. |
| 259669 | pAAV-hSyn1-EGFP-Sensor (tdT)-tTA2-WPRE | Detecting tdTomato mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct. |
| 259670 | pAAV-hSyn1-EGFP-Sensor (Th)-tTA2-WPRE | Detecting mouse tyrosine hydroxylase mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct. |
| 259671 | pAAV-hSyn1-EGFP-Sensor (cfos)-tTA2-WPRE | Detecting mouse Fos mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct. |
| 259673 | CAG-Arc | Mouse Arc (Activity-Regulated Cytoskeleton-Associated) protein is expressed under CAG promoter |
| 259778 | pAAV-hSyn1-EGFP-Sensor (vgat)-tTA2-WPRE | Detecting mouse Vgat mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct. |