pAAV-TRE3g-Nluc-RMA
(Plasmid
#259648)
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PurposeExpresses nanoluc-RMA under the TRE3g promoter.
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Depositing Lab
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Depositing OrganizationRice University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV-TRE3g-mNeon-WPRE-pA
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Backbone manufacturerJosh Huang, Addgene plasmid #192064
- Total vector size (bp) 5642
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNanoluc fused to Fc
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Alt nameNluc-RMA
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Alt nameNluc-mouse IgG1 Fc
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SpeciesM. musculus (mouse), Synthetic
- Promoter TRE3g
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttccgtaccacttcctaccc
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-TRE3g-Nluc-RMA was a gift from Jerzy Szablowski (Addgene plasmid # 259648 ; http://n2t.net/addgene:259648 ; RRID:Addgene_259648) -
For your References section:
Monitoring in vivo transcription with synthetic serum markers. Watanabe S, Lee S, Harb M, Nouraein S, Raisley E, Li H, Buitrago N, Pforr B, Szablowski JO. Nat Commun. 2026 May 27. doi: 10.1038/s41467-026-73486-2. 10.1038/s41467-026-73486-2 PubMed 42203776