pETcc/NuG2-RTX-iii-v-RS1528-NuG2
(Plasmid
#259687)
-
PurposeE. coli expression plasmid encoding CyaA RTX repeats III–V, with an RS insertion between RTX-IV and RTX-V, flanked by NuG2 domains for optical-tweezers studies.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepETcc
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameNuG2-RTX-iii-v-RS1528-NuG2
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pETcc/NuG2-RTX-iii-v-RS1528-NuG2 was a gift from Hongbin Li (Addgene plasmid # 259687 ; http://n2t.net/addgene:259687 ; RRID:Addgene_259687) -
For your References section:
The adenylate cyclase toxin RTX domain follows a series templated folding mechanism with implications for toxin activity. Chen G, Wang H, Bumba L, Masin J, Sebo P, Li H. J Biol Chem. 2023 Sep;299(9):105150. doi: 10.1016/j.jbc.2023.105150. Epub 2023 Aug 9. 10.1016/j.jbc.2023.105150 PubMed 37567473
Map uploaded by the depositor.
