The adenylate cyclase toxin RTX domain follows a series templated folding mechanism with implications for toxin activity.

Chen G, Wang H, Bumba L, Masin J, Sebo P, Li H
J Biol Chem. 2023 Sep;299(9):105150. doi: 10.1016/j.jbc.2023.105150. Epub 2023 Aug 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
259686	pETcc-NuG2-RTX-i-v-NuG2	E. coli expression plasmid encoding CyaA RTX repeats I–V flanked by NuG2 domains for single-molecule optical-tweezers studies of Ca2+-dependent RTX folding.
259687	pETcc/NuG2-RTX-iii-v-RS1528-NuG2	E. coli expression plasmid encoding CyaA RTX repeats III–V, with an RS insertion between RTX-IV and RTX-V, flanked by NuG2 domains for optical-tweezers studies.
259688	pETcc/NuG2-RTX-i-v-RS1261-NuG2	E. coli expression plasmid encoding CyaA RTX repeats I–V with an RS1261 insertion between RTX-II and RTX-III, flanked by NuG2 domains for optical-tweezers studies.
259689	pETcc/NuG2-RTX-i-v-RS1137-NuG2	E. coli expression plasmid encoding CyaA RTX repeats I–V with an RS insertion between RTX-i and RTX-ii for optical-tweezers studies of sequential RTX folding.