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pMAL-His8-MBP-3C-dscFv30
(Plasmid #259690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259690 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMAL
  • Total vector size (bp) 7483
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His8-MBP-3C-dscFv30
  • Species
    Synthetic
  • Insert Size (bp)
    1944
  • Promoter tac
  • Tags / Fusion Proteins
    • His8 (N terminal on insert)
    • Maltose Binding Protein (N terminal on insert)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer GGTCGTCAGACTGTCGATGAAGCC
  • 3′ sequencing primer TGTCCTACTCAGGAGAGCGTTCAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-His8-MBP-3C-dscFv30 was a gift from Laura Wingler (Addgene plasmid # 259690 ; http://n2t.net/addgene:259690 ; RRID:Addgene_259690)

  • For your References section:

    Angiotensin receptor conformations stabilized by biased ligands differentially modulate beta-arrestin interactions. Elgeti M, Belyaeva J, Helabad MB, Staus DP, Wingler LM. J Biol Chem. 2026 Feb;302(2):111117. doi: 10.1016/j.jbc.2025.111117. Epub 2025 Dec 29. 10.1016/j.jbc.2025.111117 PubMed 41475549
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