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pT2Apuro-mScarlet-I-ZO-1Δphos
(Plasmid #259699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259699 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    T2A
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 12556
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZO-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5956
  • Mutation
    All S or T residues in SP or TP motifs are substituted with A.
  • Entrez Gene
    TJP1 (a.k.a. ZO-1)
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • mScarlet-I (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2Apuro-mScarlet-I-ZO-1Δphos was a gift from Kazuhiro Aoki (Addgene plasmid # 259699 ; http://n2t.net/addgene:259699 ; RRID:Addgene_259699)

  • For your References section:

    ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves. Hirano S, Kondo Y, Kitajima A, Kinoshita N, Otani T, Furuse M, Ueno N, Aoki K. Nat Commun. 2026 May 9. doi: 10.1038/s41467-026-72840-8. 10.1038/s41467-026-72840-8 PubMed 42106316
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