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ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.

Hirano S, Kondo Y, Kitajima A, Kinoshita N, Otani T, Furuse M, Ueno N, Aoki K
Nat Commun. 2026 May 9. doi: 10.1038/s41467-026-72840-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
259694pCSIIpuro-EGFP-ZO-1For introducing EGFP-ZO-1 into the genome using lentivirus.
259695pCSIIpuro-EGFP-ZO-1ΔABRFor introducing EGFP-ZO-1ΔABR into the genome using lentivirus.
259696pCSIIpuro-EGFP-ZO-1ΔphosFor introducing EGFP-ZO-1Δphos into the genome using lentivirus.
259697pT2Apuro-mScarlet-I-ZO-1For introducing mScarlet-I-ZO-1 into the genome using the Tol2 transposon system.
259698pT2Apuro-mScarlet-I-ZO-1ΔABRFor introducing mScarlet-I-ZO-1ΔABR into the genome using the Tol2 transposon system.
259699pT2Apuro-mScarlet-I-ZO-1ΔphosFor introducing mScarlet-I-ZO-1Δphos into the genome using the Tol2 transposon system.
259700pCSIIhyg-mScarlet-I-KRasCTFor introducing mScarlet-I-KRasCT into the genome using lentivirus.
259701pCSIIhyg-Tks5-miRFP670For introducing Tks5-miRFP670 into the genome using lentivirus.
259702pPBhyg-Tks5-mNeonGreenFor introducing Tks5-mNeonGreen into the genome using the PiggyBac transposon system.
259703pCSIIpuro-EGFP-ZO-1ΔPDZ1For introducing EGFP-ZO-1ΔPDZ1 into the genome using lentivirus.
259704pCSIIpuro-EGFP-ZO-1ΔPDZ2For introducing EGFP-ZO-1ΔPDZ2 into the genome using lentivirus.
259705pCSIIpuro-EGFP-ZO-1ΔPDZ3For introducing EGFP-ZO-1ΔPDZ3 into the genome using lentivirus.
259706pCSIIpuro-EGFP-ZO1ΔGUKFor introducing EGFP-ZO-1ΔGUK into the genome using lentivirus.
259707pCSIIpuro-EGFP-ZO-1ΔCortactin-bindingFor introducing EGFP-ZO-1ΔCortactin-binding into the genome using lentivirus.
259708pCSIIpuro-EGFP-ZO-1_S168AFor introducing EGFP-ZO-1_S168A into the genome using lentivirus.
259709pCSIIpuro-EGFP-ZO-1_IDR2-AFor introducing EGFP-ZO-1_IDR2-A into the genome using lentivirus.
259710pCSIIpuro-EGFP-ZO-1_IDR4-1-AFor introducing EGFP-ZO-1_IDR4-1-A into the genome using lentivirus.
259711pCSIIpuro-EGFP-ZO-1_IDR4-2-AFor introducing EGFP-ZO-1_IDR4-2-A into the genome using lentivirus.
259712pCSIIpuro-EGFP-ZO1_26EFor introducing EGFP-ZO1_26E into the genome using lentivirus.
259713pCSIIpuro-EGFP-ZO-1_IDR2-EFor introducing EGFP-ZO-1_IDR2-E into the genome using lentivirus.
259714pCSIIpuro-EGFP-ZO-1_IDR4-1-EFor introducing EGFP-ZO-1_IDR4-1-E into the genome using lentivirus.
259715pCSIIpuro-EGFP-ZO-1_IDR4-2-EFor introducing EGFP-ZO-1_IDR4-2-E into the genome using lentivirus.

Antibodies from Article