pCSIIpuro-EGFP-ZO-1_IDR4-2-A
(Plasmid #259711)

• Purpose: For introducing EGFP-ZO-1_IDR4-2-A into the genome using lentivirus.
• Depositing Lab: Kazuhiro Aoki
• Depositing Organization: Kyoto University, Graduate School of Biostudies
  Located in Japan
• Publication: Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41467-026-72840-8.

Backbone

• Vector backbone: CSII
• Backbone size w/o insert (bp): 10345
• Total vector size (bp): 16335
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZO-1
• Species: H. sapiens (human)
• Insert Size (bp): 5992
• Mutation: All S/T residues in SP/TP motifs on the C-terminal side of IDR4 are substituted with A.
• Entrez Gene: TJP1 (a.k.a. ZO-1)
• Promoter: hEF1a promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry