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Addgene

MSCV KSR1 IRES GFP
(Plasmid #25973)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25973 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinase suppressor of Ras 1
  • Alt name
    KSR1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • GenBank ID
    NM_013571
  • Entrez Gene
    Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Sal1/Xho1 (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV KSR1 IRES GFP was a gift from Rob Lewis (Addgene plasmid # 25973 ; http://n2t.net/addgene:25973 ; RRID:Addgene_25973)
  • For your References section:

    The molecular scaffold KSR1 regulates the proliferative and oncogenic potential of cells. Kortum RL, Lewis RE. Mol Cell Biol. 2004 May . 24(10):4407-16. 10.1128/MCB.24.10.4407-4416.2004 PubMed 15121859