RP165 USP7 R152C MycTag
(Plasmid
#259782)
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PurposeExpresses human full length mutant R152C USP7 with a N-terminal Myc tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4353
- Total vector size (bp) 7661
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUSP7 (Ubiquitin carboxyl-terminal hydrolase 7)
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Alt nameHAUSP
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SpeciesH. sapiens (human)
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Insert Size (bp)3308
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MutationR152C
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Entrez GeneUSP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
- Promoter CMVIE94 promoter
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Tag
/ Fusion Protein
- N-terminal Myc tag (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.04.24.720667 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RP165 USP7 R152C MycTag was a gift from Irina Bezsonova (Addgene plasmid # 259782 ; http://n2t.net/addgene:259782 ; RRID:Addgene_259782)