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pET15b His-FAF1 Helix-UBX (481-650)
(Plasmid #259890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259890 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FAF1
  • Species
    H. sapiens (human)
  • Mutation
    deleted aa 1-480
  • Entrez Gene
    FAF1 (a.k.a. CGI-03, HFAF1s, UBXD12, UBXN3A, hFAF1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Prescission protease cleavage site after His-tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b His-FAF1 Helix-UBX (481-650) was a gift from Hemmo Meyer (Addgene plasmid # 259890 ; http://n2t.net/addgene:259890 ; RRID:Addgene_259890)

  • For your References section:

    The accessory adapters FAF1, FAF2, and UBXN7 accelerate proteasomal degradation by increasing prior p97-mediated substrate unfolding. Kracht M, Kroning A, van den Boom J, Gahlot P, Koska S, Kiss L, Meyer H. Sci Adv. 2026 Mar 6;12(10):eaea7381. doi: 10.1126/sciadv.aea7381. Epub 2026 Mar 6. 10.1126/sciadv.aea7381 PubMed 41790892
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