The accessory adapters FAF1, FAF2, and UBXN7 accelerate proteasomal degradation by increasing prior p97-mediated substrate unfolding.
Kracht M, Kroning A, van den Boom J, Gahlot P, Koska S, Kiss L, Meyer H
Sci Adv. 2026 Mar 6;12(10):eaea7381. doi: 10.1126/sciadv.aea7381. Epub 2026 Mar 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 259888 | pET28a His-FAF1 | Express human FAF1 with an N-terminal His-tag |
| 259889 | pET28a His-FAF1 ΔN (Δ1-270) | Express human FAF1 with an N-terminal His-tag, first 270 aa missing |
| 259890 | pET15b His-FAF1 Helix-UBX (481-650) | Express human FAF1 with an N-terminal His-tag, aa 481-650 |
| 259891 | pET28a His-FAF1 UBX (569-650) | Express UBX domain of human FAF1 with an N-terminal His-tag |
| 259892 | pET28a His-FAF1 V507A K508A | Express human FAF1 with an N-terminal His-tag, V507A K508A mutations |
| 259893 | pET28a His-FAF2 ΔTMD (Δ90-118) | Express human FAF2 with an N-terminal His-tag, without Transmembrane domain |
| 259894 | pET28a His-FAF2 ΔN (Δ1-131) | Express human FAF2 with an N-terminal His-tag, first 131 aa missing |
| 259895 | pET28a His-FAF2 Helix-UBX (276-445) | Express human FAF2 with an N-terminal His-tag, aa 276-445 |
| 259896 | pET28a His-FAF2 UBX (350-445) | Express UBX domain of human FAF2 with an N-terminal His-tag |
| 259897 | pET28a His-FAF2 ΔTMD L289A R290A | Express human FAF2 with an N-terminal His-tag, without Transmembrane domain, L289A R290A mutations |
| 259898 | pET28a His-UBXN7 | Express human UBXN7 with an N-terminal His-tag |
| 259899 | pET28a His-UBXN7 ΔUBA (Δ2-54) | Express human UBXN7 with an N-terminal His-tag, aa 2-54 missing |
| 259900 | pET28a His-UBXN7 ΔN (Δ1-100) | Express human UBXN7 with an N-terminal His-tag, first 100 aa missing |
| 259901 | pET28a His-UBXN7 Linker-UBX (Δ1-265) | Express human UBXN7 with an N-terminal His-tag, first 265 aa missing |
| 259902 | pET41 His-SUMO-Ub4-mEos3.2-Intein-CBD | Express Tetra-ubiquitin-mEos3.2 construct, with N-terminal His-SUMO and C-terminal Intein-CBD tags |
| 259903 | pET41 His-SUMO-Ub4-mEos3.2-Tail-Intein-CBD | Express Tetra-ubiquitin-mEos3.2-Tail construct, with N-terminal His-SUMO and C-terminal Intein-CBD tags |
| 259904 | pGEX6P1 GST-Rad23B | Express human Rad23B, with N-terminal GST-tag |