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3-in-1 GzESTY
(Plasmid #259906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259906 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 259906-D50 50 μg of DNA in Tris buffer $495
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DNA 259906-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC57
  • Total vector size (bp) 10346
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GloSensor
  • Species
    Synthetic
  • Insert Size (bp)
    2172

Gene/Insert 2

  • Gene/Insert name
    PTX-S1
  • Species
    Bordetella Pertussis
  • Insert Size (bp)
    807

Gene/Insert 3

  • Gene/Insert name
    GsGz chimera
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1182

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The sequence encoding the GloSensor was obtained from Promega (MTA) and the first aa after the initial methionine was changed to introduce a Kozak sequence (Pro to Ala). We obtained permission to slightly modify the sequence.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GsGz chimera was a gift from Robert Lucas (Addgene plasmid #109355)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3-in-1 GzESTY was a gift from Cesare Orlandi (Addgene plasmid # 259906 ; http://n2t.net/addgene:259906 ; RRID:Addgene_259906)

  • For your References section:

    G(z)ESTY as an optimized cell-based assay for initial steps in GPCR deorphanization. Franchini L, Porter JJ, Lueck JD, Orlandi C. Nat Commun. 2025 May 15;16(1):4521. doi: 10.1038/s41467-025-59850-8. 10.1038/s41467-025-59850-8 PubMed 40374633
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