4-in-1 ADRA2A-GzESTY
(Plasmid #259908)

• Purpose: Mammalian expression of the following 4 proteins: GsGz, PTX(S1), GloSensor-22F, and ADRA2AR.
• Depositing Lab: Cesare Orlandi
• Depositing Organization: University of Rochester Medical Center
  Located in the United States of America
• Publication: Franchini et al Nat Commun. 2025 May 15;16(1):4521. doi: 10.1038/s41467-025-59850-8.

Backbone

• Vector backbone: pUC57
• Total vector size (bp): 13489
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GloSensor
• Species: Synthetic
• Insert Size (bp): 2172

Gene/Insert 2

• Gene/Insert name: PTX-S1
• Species: Bordetella Pertussis
• Insert Size (bp): 807

Gene/Insert 3

• Gene/Insert name: adra2a
• Species: H. sapiens (human)
• Insert Size (bp): 1395
• Entrez Gene: ADRA2A (a.k.a. ADRA2, ADRA2R, ADRAR, ALPHA2AAR, FPLD8)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Gene/Insert 4

• Gene/Insert name: GsGz chimera
• Species: H. sapiens (human)
• Insert Size (bp): 1182

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The GsGz chimera was a gift from Robert Lucas (Addgene plasmid #109355)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • GloSensor™ Vector Limited Use Label License
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 259908-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • GloSensor™ Vector Limited Use Label License
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 259908-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • GloSensor™ Vector Limited Use Label License
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.