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pDY2365 pCMV-PE2-Bxb1_v12k_q226k_h334k (enPASTE)
(Plasmid #260029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260029 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    CRISPR ; Recombinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pCMV-PE2-P2A-Bxb1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, finding R5K in the first internal SV40 NLS in the insert ORF. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDY2365 pCMV-PE2-Bxb1_v12k_q226k_h334k (enPASTE) was a gift from Jonathan Gootenberg (Addgene plasmid # 260029 ; http://n2t.net/addgene:260029 ; RRID:Addgene_260029)

  • For your References section:

    Structure and engineering of the large serine recombinase Bxb1 for gene integration. Soma T, Hiraizumi M, Fell CW, Tagliaferri D, Lequyer J, Okazaki S, Isayama Y, Kato K, Sapkota S, Ramani H, Arya B, Schmitt-Ulms C, Yamashita K, Gootenberg JS, Abudayyeh OO, Nishimasu H. Mol Cell. 2026 Jun 18;86(12):2247-2262.e7. doi: 10.1016/j.molcel.2026.05.018. Epub 2026 Jun 8. 10.1016/j.molcel.2026.05.018 PubMed 42259299
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