Hs PEG10-Dendra2 gag-pol isoform3
(Plasmid
#260100)
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PurposeExpression of Human PEG10-dendra2 isoform3 and CFP using IRES
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePEG10
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Alt namepAMW117
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SpeciesH. sapiens (human)
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Insert Size (bp)4445
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Entrez GenePEG10 (a.k.a. EDR, HB-1, MEF3L, Mar2, Mart2, RGAG3, RTL2, SIRH1)
- Promoter CMV
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Tag
/ Fusion Protein
- dendra2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hs PEG10-Dendra2 gag-pol isoform3 was a gift from Alexandra Whiteley (Addgene plasmid # 260100 ; http://n2t.net/addgene:260100 ; RRID:Addgene_260100) -
For your References section:
UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS. Black HH, Hanson JL, Roberts JE, Leslie SN, Campodonico W, Ebmeier CC, Holling GA, Tay JW, Matthews AM, Ung E, Lau CI, Whiteley AM. Elife. 2023 Mar 23;12:e79452. doi: 10.7554/eLife.79452. 10.7554/eLife.79452 PubMed 36951542