Mm HA-Peg10 gag-pol
(Plasmid
#260101)
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PurposeExpression of Mouse 2x HA-tagged PEG10
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePEG10
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Alt namepAMW114
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SpeciesM. musculus (mouse)
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Insert Size (bp)2990
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Entrez GenePeg10 (a.k.a. Edr, HB-1, MEF3L, Mar2, Mart2, MmPEG10, MyEF-3, Rtl2)
- Promoter CMV
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Tag
/ Fusion Protein
- 2x HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mm HA-Peg10 gag-pol was a gift from Alexandra Whiteley (Addgene plasmid # 260101 ; http://n2t.net/addgene:260101 ; RRID:Addgene_260101) -
For your References section:
Global proteomics of Ubqln2-based murine models of ALS. Whiteley AM, Prado MA, de Poot SAH, Paulo JA, Ashton M, Dominguez S, Weber M, Ngu H, Szpyt J, Jedrychowski MP, Easton A, Gygi SP, Kurz T, Monteiro MJ, Brown EJ, Finley D. J Biol Chem. 2021 Jan-Jun;296:100153. doi: 10.1074/jbc.RA120.015960. Epub 2020 Dec 10. 10.1074/jbc.RA120.015960 PubMed 33277362