VAPA-Scarlet_pJWM_032
(Plasmid
#260156)
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PurposeIntestine expression of VAPA/F53B6.4 tagged with WormScarlet: ges-1p::F53B6.4-2xLinker-WormScarlet::SL2WormGreenLantern::unc-54 3’UTR
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGGL3691_003
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameF53B6.4-Scarlet
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SpeciesC. elegans (nematode)
- Promoter ges-1p
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer SP6: ATTTAGGTGACACTATAGA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VAPA-Scarlet_pJWM_032 was a gift from Anne Brunet (Addgene plasmid # 260156 ; http://n2t.net/addgene:260156 ; RRID:Addgene_260156) -
For your References section:
An in vivo resource of age-regulated C. elegans intestinal secretory-pathway proteins highlights secreted enzymes associated with lifespan regulation. Miklas JW, Papsdorf K, Sun ED, Tsenter A, Haseley NR, Ameglio B, Medoh UN, Richard D, McCarthy F, Artiles KL, Hims A, Levina A, Zhou OY, Elias JE, Abu-Remaileh M, Brunet A. Cell Rep. 2026 Aug 27;45(9):117902. doi: 10.1016/j.celrep.2026.117902. 10.1016/j.celrep.2026.117902 PubMed 42658673