pKLV2.2-mU6epegRNA(SapI)-hU6epegRNA(BbsI)-PGKpuroBFP-W
(Plasmid
#260204)
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Purpose(Empty Backbone) Empty vector for dual pegRNA expression that can be cloned in individually using either SalI or BbsI
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Depositing Lab
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Depositing OrganizationWellcome Sanger Institute
Located in the United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepKLV2 lentiviral vector
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Vector typeLentiviral, CRISPR, Synthetic Biology
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- BFP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- BFP (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKLV2.2-mU6epegRNA(SapI)-hU6epegRNA(BbsI)-PGKpuroBFP-W was a gift from Leopold Parts (Addgene plasmid # 260204 ; http://n2t.net/addgene:260204 ; RRID:Addgene_260204)
