Leopold Parts Lab: Parts Lab Plasmids 2026
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260187 | pB-CAGGS-dCas9-KRAB-MeCP2-BSD-mScarlet | Expressed dCas9 linked with MECP2 and KRAB domain for CRISPRi screening and has blasticidin and mScarlet markers all in a piggyBac cassette |
| 260189 | pPB_TREG3G-PE7-rtTA3G-P2A-eGFP | Piggybac vector with doxycyclin-inducible prime editor for mammalian cells |
| 260190 | Cas3-Cascade-all-in-one-BSD | All in one Cas3 and Cascade expression plasmid with blasticidin selection marker |
| 260191 | Inducible-Cas3-Cascade-all-in-one-BSD | Doxycyline inducible all-in-one Cas3 and Cascade expression plasmid with blasticidin selection marker |
| 260192 | pKLV2-U6-crRNA-BbsI-PGKpuroBFP-W | Lentiviral backbone to clone Cas3 crRNAs into with BFP expression and puromycin resistance |
| 260194 | pCS2-puro-T2A-BFP | Encodes for puromycin resistance and BFP, perfect for transfection control |
| 260195 | pCS2-puro-T2A-GFP | Encodes for puromycin resistance and GFP, perfect for transfection control |
| 260196 | pCS2-puro-T2A-mCherry | Encodes for puromycin resistance and mCherry, perfect for transfection control |
| 260198 | pCS2-blasticidin-T2A-GFP | Encodes for blasticidin resistance and GFP, perfect for transfection control |
| 260199 | pCS2-blasticidin-T2A-mCherry | Encodes for blasticidin resistance and mCherry, perfect for transfection control |
| 260204 | pKLV2.2-mU6epegRNA(SapI)-hU6epegRNA(BbsI)-PGKpuroBFP-W | Empty vector for dual pegRNA expression that can be cloned in individually using either SalI or BbsI |
| 260208 | pLenti-Puro-T2A-mCherry | Lentiviral backbone to clone pegRNA with puromycin resistance and mCherry expression |
| 260209 | pLenti-ePegRNA-acceptor-mCherry | Lentiviral backbone to clone pegRNA with puromycin resistance and mCherry expression, encodes for mRFP for cloning selection |
| 260210 | pLenti-ePegRNA-acceptor-BFP | Lentiviral backbone to clone pegRNA with puromycin resistance and BFP expression, encodes for mRFP for cloning selection |
| 260211 | pLenti-puro-mCherry-epeg | Lentiviral backbone to clone pegRNA with tevopepQ and puromycin resistance and mCherry expression |
| 260212 | pLenti-puro-mCherry-terminator | Lentiviral backbone to clone pegRNA and puromycin resistance and mCherry expression |
| 260213 | Pa01_CARGO_swappedAttP_insertTest_eGFP | Donor plasmid to test for GFP integration with Pa01 |