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pLenti-ePegRNA-acceptor-mCherry
(Plasmid #260209)

  • Purpose
    (Empty Backbone) Lentiviral backbone to clone pegRNA with puromycin resistance and mCherry expression, encodes for mRFP for cloning selection
  • Depositing Lab
  • Depositing Organization
    Wellcome Sanger Institute
    Located in the United Kingdom of Great Britain and Northern Ireland
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260209 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pKLV2 lentiviral vector
  • Vector type
    CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-ePegRNA-acceptor-mCherry was a gift from Leopold Parts (Addgene plasmid # 260209 ; http://n2t.net/addgene:260209 ; RRID:Addgene_260209)