Cas3-Cascade-all-in-one-BSD
(Plasmid
#260190)
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PurposeAll in one Cas3 and Cascade expression plasmid with blasticidin selection marker
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Depositing Lab
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Depositing OrganizationWellcome Sanger Institute
Located in United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPV
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
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Alt nameBlasticidin
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SpeciesE. Coli
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cas3-Cascade-all-in-one-BSD was a gift from Leopold Parts (Addgene plasmid # 260190 ; http://n2t.net/addgene:260190 ; RRID:Addgene_260190)