pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep
(Plasmid
#260206)
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PurposePlasmid encoding for GFP and BFP with a CRISPRi gRNA targeting GFP to validate CRISPRi activity
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Depositing Lab
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Depositing OrganizationWellcome Sanger Institute
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCS2+
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Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP targeting gRNA
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Alt nameGFP, BFP
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gRNA/shRNA sequenceUnspecified
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SpeciesSynthetic
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep was a gift from Leopold Parts (Addgene plasmid # 260206 ; http://n2t.net/addgene:260206 ; RRID:Addgene_260206) -
For your References section:
Optimized whole-genome CRISPR interference screens identify ARID1A-dependent growth regulators in human induced pluripotent stem cells. Usluer S, Hallast P, Crepaldi L, Zhou Y, Urgo K, Dincer C, Su J, Noell G, Alasoo K, El Garwany O, Gerety SS, Newman B, Dovey OM, Parts L. Stem Cell Reports. 2023 May 9;18(5):1061-1074. doi: 10.1016/j.stemcr.2023.03.008. Epub 2023 Apr 6. 10.1016/j.stemcr.2023.03.008 PubMed 37028423
