Optimized whole-genome CRISPR interference screens identify ARID1A-dependent growth regulators in human induced pluripotent stem cells.
Usluer S, Hallast P, Crepaldi L, Zhou Y, Urgo K, Dincer C, Su J, Noell G, Alasoo K, El Garwany O, Gerety SS, Newman B, Dovey OM, Parts L
Stem Cell Reports. 2023 May 9;18(5):1061-1074. doi: 10.1016/j.stemcr.2023.03.008. Epub 2023 Apr 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260206 | pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep | Plasmid encoding for GFP and BFP with a CRISPRi gRNA targeting GFP to validate CRISPRi activity |
| 260207 | pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock | Plasmid encoding for GFP and BFP with a non-targeting CRISPRi gRNA to validate CRISPRi activity |