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pUF-dCas12-BSD-Sir2a
(Plasmid #260380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260380 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUF
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 12316
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas12
  • Alt name
    human codon-optimized dead (832A) LbCpf1
  • Species
    Lachnospiraceae bacterium
  • Insert Size (bp)
    3684
  • Mutation
    832A
  • Promoter Pfhsp86 5" UTR

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUF-dCas12-BSD-Sir2a was a gift from Liwang Cui (Addgene plasmid # 260380 ; http://n2t.net/addgene:260380 ; RRID:Addgene_260380)

  • For your References section:

    Inducible dCas12a system expands CRISPRi/a capabilities for functional genomics in malaria parasite Plasmodium falciparum. Lucky AB, Li XL, Kobakhidze G, Gonzales JL, Wang C, Cui L, Dong G, Miao J. iScience, 22 Sep 2026, 117704 10.1016/j.isci.2026.117704
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