UPRT-mCh-Nluc-P2A-neo-UPRT
(Plasmid #135015)

• Purpose: Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus
• Depositing Lab: David Sibley
• Publication: Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S1931-3128(19)30252-5. doi: 10.1016/j.chom.2019.05.007.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 2300
• Total vector size (bp): 8357
• Modifications to backbone: No
• Vector type: Cryptosporidium Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCh-Nluc-P2A-neo
• Species: Synthetic
• Promoter: Cryptosporidium actin and enolase
• Tag / Fusion Protein:
  • mCherry and Nluc-neo

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TGAGAGCTTTCAATCAGCTCAGAATGAGTTGGTTATAAACAGT
• 3′ sequencing primer: GTTATAGATTGCATGCGCTTAATTAATCAGAAGAATTCGTCAAGA

Gene/Insert 2

• Gene/Insert name: UPRT 5'UTR
• Species: Cryptosporidium parvum

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ttacgccaagcttgcatgcctgcagagttataagcacca
• 3′ sequencing primer: ataaccaactcattctgagctgattgaaagctctcaattgagt

Gene/Insert 3

• Gene/Insert name: UPRT 3'UTR
• Species: Cryptosporidium parvum

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: attcttctgattaattaagcgcatgcaatctataactaattccaaa
• 3′ sequencing primer: cagtgaattcgagctcggtacccgggattatacttccttgctggtt

Resource Information

• Supplemental Documents:
  • UPRT-mCh-Nluc-P2A-neo-UPRT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry