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A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
Wilke G, Funkhouser-Jones LJ, Wang Y, Ravindran S, Wang Q, Beatty WL, Baldridge MT, VanDussen KL, Shen B, Kuhlenschmidt MS, Kuhlenschmidt TB, Witola WH, Stappenbeck TS, Sibley LD
Cell Host Microbe. 2019 Jun 18. pii: S1931-3128(19)30252-5. doi: 10.1016/j.chom.2019.05.007.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122852pACT1:Cas9-GFP, U6:sgTKExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene
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122853pACT1:Cas9-GFP, U6:sgUPRTExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
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134896TK-GFP-Nluc-P2A-neo-TKExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus
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135015UPRT-mCh-Nluc-P2A-neo-UPRTExpresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus
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