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pSH4-2/Stop-flox-mCherry
(Plasmid #260580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260580 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    SH4-2 Targeting Vector
  • Backbone manufacturer
    Marius Wernig, described by Uenaka et al. 2026, https://doi.org/10.1016/j.stem.2026.01.007
  • Total vector size (bp) 12125
  • Modifications to backbone
    LoxStopLox mCherry cassette replaces mScarlet/mNeon Green
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    711

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH4-2/Stop-flox-mCherry was a gift from Bill Skarnes (Addgene plasmid # 260580 ; http://n2t.net/addgene:260580 ; RRID:Addgene_260580)

  • For your References section:

    A Xeno-Free Protocol for Rapid Differentiation of Human iPSC-Derived Microglia from the KOLF2.1J Reference Line. Oliveira NAJ, Lewkowicz KR, Clow PA, Ward ME, Cookson MR, Skarnes WC, McDonough JA. Bioengineering (Basel). 2025 Dec 30;13(1):45. doi: 10.3390/bioengineering13010045. 10.3390/bioengineering13010045 PubMed 41595977
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