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A Xeno-Free Protocol for Rapid Differentiation of Human iPSC-Derived Microglia from the KOLF2.1J Reference Line.

Oliveira NAJ, Lewkowicz KR, Clow PA, Ward ME, Cookson MR, Skarnes WC, McDonough JA
Bioengineering (Basel). 2025 Dec 30;13(1):45. doi: 10.3390/bioengineering13010045. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
260580pSH4-2/Stop-flox-mCherrySH4-2 donor plasmid encoding a CAG promoter, a loxP-flanked STOP cassette, and mCherry. Includes WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) sequences.

Antibodies from Article