pv6_BRD4(1)_BRD.1x
(Plasmid
#260595)
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PurposeExpresses the first bromodomain from BRD4 fused to eGFP including flexible linker and NLS
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Depositing Lab
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Depositing OrganizationUtrecht University
Located in the Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepv6
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRD4(1)_BRD.1x
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SpeciesM. musculus (mouse)
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MutationWT
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Entrez GeneBrd4 (a.k.a. Brd5, HUNK1, MCAP, WI-11513)
- Promoter CAGGS
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2025.09.06.674632v1 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pv6_BRD4(1)_BRD.1x was a gift from Tuncay Baubec (Addgene plasmid # 260595 ; http://n2t.net/addgene:260595 ; RRID:Addgene_260595)