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Tuncay Baubec Lab: A modular toolbox for in cellulo screening of small-molecule inhibitors targeting chromatin reader domains

Unpublished

Plasmids from Article

ID Plasmid Purpose
260585pv6_BAZ2A_BRD.1xExpresses bromodomain from BAZ2a fused to eGFP including flexible linker and NLS
260586pv6_BRDT_BRD.1xExpresses bromodomain from BRDT fused to eGFP including flexible linker and NLS
260587pv6_CECR2_BRD.1xExpresses bromodomain from CECR2 fused to eGFP including flexible linker and NLS
260588pv6_P300_BRD.1xExpresses bromodomain from p300 fused to eGFP including flexible linker and NLS
260589pv6_SMARCA2_BRD.1xExpresses bromodomain from SMARCA2a fused to eGFP including flexible linker and NLS
260590pv6_TAF1(2)_BRD.1xExpresses the first bromodomain from TAF1 fused to eGFP including flexible linker and NLS
260591pv6_TRIM28_BRD.1xExpresses bromodomain from TRIM28 fused to eGFP including flexible linker and NLS
260593pv6_BRD4(1)_BRD.1x_mutant_N140FExpresses a binding-deficient mutant of the first bromodomain from BRD4 fused to eGFP including flexible linker and NLS
260594pv6_BRD4(2)_BRD.1x_mutant_N434FExpresses a binding-deficient mutant of the second bromodomain from BRD4 fused to eGFP including flexible linker and NLS
260595pv6_BRD4(1)_BRD.1xExpresses the first bromodomain from BRD4 fused to eGFP including flexible linker and NLS
260596pv6_BRD4(2)_BRD.1xExpresses the second bromodomain from BRD4 fused to eGFP including flexible linker and NLS
260597pv6_BRD4(2)_BRD.2xExpresses two copies of the second bromodomain from BAZ2a fused to eGFP including flexible linker and NLS
260598pv6_CBP_BRD.1xExpresses bromodomain from CBP fused to eGFP including flexible linker and NLS
260600pv6_BRD4(1_2)_BRD.1xExpresses the first and second bromodomains from BRD4 fused to eGFP including flexible linker and NLS
260603pv6_BRD9_BRD.1xExpresses bromodomain from BRD9 fused to eGFP including flexible linker and NLS
260604pv6_BRD9_BRD.2xExpresses two copies of the bromodomain from BRD9 fused to eGFP including flexible linker and NLS
260605pv6_BRD2_BRD.1xExpresses bromodomain from BRD2 fused to eGFP including flexible linker and NLS
260606pv6_BRD3_BRD.1xExpresses bromodomain from BRD3 fused to eGFP including flexible linker and NLS
260607pv6_SMARCA4_BRD.1xExpresses bromodomain from SMARCA4 fused to eGFP including flexible linker and NLS

Antibodies from Article