pExpress_ubi-bOpto-fgf
(Plasmid
#260611)
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PurposeTol2-compatible plasmid containing ubi:bOpto-FGF and transgenesis marker exorh:mCherry
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260611 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepExpress
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Vector typeTol2 transgenesis vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebOpto-FGF
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SpeciesD. rerio (zebrafish); Vaucheria frigida
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Insert Size (bp)1893
Resource Information
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A portion of this plasmid was derived from a plasmid made bybOpto-FGF was originally described in Iannucci, Maria Thomas, et al. (2026) Dev. Biol. (PMC13215188). ubi promoter is from Mosimann et al. (2011) Dev. (PMC2998170). exorh promoter is from Kemmler et al. (2023) Dev. (PMC10263156).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExpress_ubi-bOpto-fgf was a gift from Katherine Rogers (Addgene plasmid # 260611 ; http://n2t.net/addgene:260611 ; RRID:Addgene_260611) -
For your References section:
A transgenic zebrafish for spatiotemporal optogenetic activation of FGF/ERK signaling. Anderson WK, Iannucci LE, Sinaii N, Porcino J, Rogers KW. Biol Open. 2026 Sep 15;15(9):bio062695. doi: 10.1242/bio.062695. Epub 2026 Sep 9. 10.1242/bio.062695 PubMed 42713714