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PKCdelta KD (K378R)
(Plasmid #260717)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260717 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pINDUCER21-puro_Gateway-3xFLAG
  • Backbone manufacturer
    Michael Wehr (Addgene Plasmid #172981)
  • Backbone size w/o insert (bp) 10480
  • Total vector size (bp) 12508
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKCD KD (K378R)
  • Alt name
    PKCdelta Kinase Dead (K378R)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2025
  • Mutation
    K378R
  • GenBank ID
    NP_001303256.1
  • Entrez Gene
    PRKCD (a.k.a. ALPS3, CVID9, MAY1, PKCD, nPKC-delta)
  • Promoter tetracycline response element
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.27.671140 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKCdelta KD (K378R) was a gift from William Kaelin (Addgene plasmid # 260717 ; http://n2t.net/addgene:260717 ; RRID:Addgene_260717)

  • For your References section:

    Positive selection screen identifies natural product beta-catenin inactivators. Boudreau MW, Freire VF, Corbett SC, Martinez-Fructuoso L, Shenoy SR, Yu W, Kumar R, Thornburg CC, Akee RK, Peyser BD, Jiang Q, Splaine J, Pfaff JL, Yu D, Chandler BC, Abeja DM, Donovan KA, Che J, Lampson BL, Cooke M, Kazanietz MG, Szajner P, Smith JA, Koduri V, Grkovic T, O'Keefe BR, Kaelin WG Jr. Proc Natl Acad Sci U S A. 2026 Sep 29;123(39):e2625189123. doi: 10.1073/pnas.2625189123. Epub 2026 Sep 25. 10.1073/pnas.2625189123 PubMed 42789292
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