Positive selection screen identifies natural product beta-catenin inactivators.
Boudreau MW, Freire VF, Corbett SC, Martinez-Fructuoso L, Shenoy SR, Yu W, Kumar R, Thornburg CC, Akee RK, Peyser BD, Jiang Q, Splaine J, Pfaff JL, Yu D, Chandler BC, Abeja DM, Donovan KA, Che J, Lampson BL, Cooke M, Kazanietz MG, Szajner P, Smith JA, Koduri V, Grkovic T, O'Keefe BR, Kaelin WG Jr
Proc Natl Acad Sci U S A. 2026 Sep 29;123(39):e2625189123. doi: 10.1073/pnas.2625189123. Epub 2026 Sep 25.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260712 | beta-catenin S37C IRES GFP | Expression of a bicistronic cDNA encoding beta-catenin S37C (V5 tagged) and EGFP |
| 260713 | beta-catenin S37C-DCK* IRES GFP | Expression of a bicistronic cDNA encoding beta-catenin S37C fused to modified deoxycytidine kinase (DCK*, V5 tagged) and EGFP |
| 260714 | beta-catenin S37C/S715A-DCK* IRES GFP | Expression of a bicistronic cDNA encoding beta-catenin S37C/S715A fused to modified deoxycytidine kinase (DCK*, V5 tagged) and EGFP |
| 260715 | beta-catenin (1-665) S37C-DCK* IRES GFP | Expression of a bicistronic cDNA encoding the 1-665 amino acids of beta-catenin S37C/ fused to modified deoxycytidine kinase (DCK*, V5 tagged) and EGFP |
| 260716 | PKCdelta WT | DOX inducible expression of PKCdelta WT (FLAG-tagged) |
| 260717 | PKCdelta KD (K378R) | DOX inducible expression of PKCdelta KD K378R (FLAG-tagged). KD: kinase-dead |
| 260718 | PKCdelta CA (R144A/R145A) | DOX inducible expression of PKCdelta CA R144A/R145A (FLAG-tagged). CA: constitutively active |
| 260719 | PKCtheta WT | DOX inducible expression of PKCtheta WT (FLAG-tagged) |
| 260720 | PKCtheta KD (K409R) | DOX inducible expression of PKCtheta KD K409R (FLAG-tagged). KD: kinase-dead |