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pCBA.opI-SceI
(Plasmid #260786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260786 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Ampicillin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized meganuclease I-SceI

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon-optimized I-SceI shows 2-fold higher expression in HEK293 cells than Addgene plasmid #26477.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBA.opI-SceI was a gift from Dan Wang (Addgene plasmid # 260786 ; http://n2t.net/addgene:260786 ; RRID:Addgene_260786)

  • For your References section:

    Viral gene replication enhances AAV vector quality and reduces manufacturing costs. Liu H, Du A, Liu N, Kapadia M, Chen X, Liang J, Lee JS, Xie J, Gao G, Wang D. Mol Ther. 2026 Aug 11:S1525-0016(26)00689-1. doi: 10.1016/j.ymthe.2026.08.008. 10.1016/j.ymthe.2026.08.008 PubMed 42581546
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