Viral gene replication enhances AAV vector quality and reduces manufacturing costs.
Liu H, Du A, Liu N, Kapadia M, Chen X, Liang J, Lee JS, Xie J, Gao G, Wang D
Mol Ther. 2026 Aug 11:S1525-0016(26)00689-1. doi: 10.1016/j.ymthe.2026.08.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260785 | TP.EGFP | Template plasmid expressing I-SceI site- and Ad5 ITR-flanked EGFP (pHL300d) |
| 260786 | pCBA.opI-SceI | Codon-optimized meganuclease I-SceI driven by CBA promoter (pHL402b) |
| 260791 | RP 3.0 | Replication plasmid version 3.0 expressing Ad5 polymerase, pTP and codon-optimized I-SceI (pHL426b) |
| 260796 | TP.Rep2/Cap2-NF1A.EGFP | Template plasmid expressing I-SceI site- and Ad5 ITR-flanked Rep2/Cap2-NF1A and AAV ITR-flanked EGFP (pHL492) |
| 260798 | TP.Rep2/Cap2-NF1A.GPR78 | Template plasmid expressing I-SceI site- and Ad5 ITR-flanked Rep2/Cap2-NF1A and AAV ITR-flanked GPR78 (pALD18) |