pBAD-ratioCitron1Low
(Plasmid
#260800)
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PurposeBacterial expression of low affinity excitation ratiometric citrate biosensor ratioCitron1Low
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Depositing Lab
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Depositing OrganizationThe University of Tokyo
Located in Japan -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameratioCitron1Low
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SpeciesSynthetic
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Insert Size (bp)1107
- Promoter pBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.64898/2026.04.16.718871v1 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-ratioCitron1Low was a gift from Robert Campbell (Addgene plasmid # 260800 ; http://n2t.net/addgene:260800 ; RRID:Addgene_260800) -
For your References section:
Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity. Hario S, Tamura N, Alladin-Mustan BS, Ali SM, Macauley MS, Shen Y, Campbell RE, Huppertz I, Takahashi-Yamashiro K. bioRxiv 2026.04.16.718871 10.64898/2026.04.16.718871