Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity

Hario S, Tamura N, Alladin-Mustan BS, Ali SM, Macauley MS, Shen Y, Campbell RE, Huppertz I, Takahashi-Yamashiro K
bioRxiv 2026.04.16.718871 Article

Plasmids from Article

ID	Plasmid	Purpose
260800	pBAD-ratioCitron1Low	Bacterial expression of low affinity excitation ratiometric citrate biosensor ratioCitron1Low
260801	pBAD-ratioCitron1High	Bacterial expression of high affinity excitation ratiometric citrate biosensor ratioCitron1High
260802	pBAD-ratioCitron1Low-con	Bacterial expression of low affinity excitation ratiometric citrate control biosensor ratioCitron1Low-con
260803	pBAD-ratioCitron1High-con	Bacterial expression of high affinity excitation ratiometric citrate control biosensor ratioCitron1High-con
260804	pcDNA3-ratioCitron1Low	Mammalian expression of low affinity excitation ratiometric citrate biosensor ratioCitron1Low
260805	pcDNA3-ratioCitron1High	Mammalian expression of high affinity excitation ratiometric citrate biosensor ratioCitron1High
260806	pcDNA3-ratioCitron1Low-con	Mammalian expression of low affinity excitation ratiometric citrate control biosensor ratioCitron1Low-con
260807	pcDNA3-ratioCitron1High-con	Mammalian expression of high affinity excitation ratiometric citrate control biosensor ratioCitron1High-con
260808	pcDNA3-mito-ratioCitron1Low	Mammalian expression of mitochondria-targeted low affinity excitation ratiometric citrate biosensor ratioCitron1Low
260809	pcDNA3-mito-ratioCitron1High	Mammalian expression of mitochondria-targeted high affinity excitation ratiometric citrate biosensor ratioCitron1High
260810	pcDNA3-mito-ratioCitron1Low-con	Mammalian expression of mitochondria-targeted low affinity excitation ratiometric citrate control biosensor ratioCitron1Low-con
260811	pcDNA3-mito-ratioCitron1High-con	Mammalian expression of mitochondria-targeted high affinity excitation ratiometric citrate control biosensor ratioCitron1High-con