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pGEX-6P1-SPRTN-MPD (26-150)
(Plasmid #260837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260837 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEX-6P1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPRTN
  • Alt name
    C1orf124
  • Alt name
    DVC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    375
  • Entrez Gene
    SPRTN (a.k.a. C1orf124, DVC1, PRO4323, spartan)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GST tag can be removed by HRV-3C protease.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1-SPRTN-MPD (26-150) was a gift from Kristijan Ramadan (Addgene plasmid # 260837 ; http://n2t.net/addgene:260837 ; RRID:Addgene_260837)

  • For your References section:

    DNA-induced conformational changes in SPRTN relieve its auto-inhibitory effect on protease activity. Song W, Newman JA, Zhao Y, Chalk R, Redfield C, Elliott PR, Ramadan K. bioRxiv [Preprint]. 2026 May 1:2026.04.29.721615. doi: 10.64898/2026.04.29.721615. 10.64898/2026.04.29.721615 PubMed 42094449
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