DNA-induced conformational changes in SPRTN relieve its auto-inhibitory effect on protease activity.
Song W, Newman JA, Zhao Y, Chalk R, Redfield C, Elliott PR, Ramadan K
bioRxiv [Preprint]. 2026 May 1:2026.04.29.721615. doi: 10.64898/2026.04.29.721615.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260827 | pET-24-hexaUb | Expression in E. coli of linear hexa-ubiquitin. |
| 260828 | pET-24-octaUb | Expression in E. coli of linear octa-ubiquitin. |
| 260830 | pNIC28-SPRTN (1-227) | Expression in E. coli of SPRTN 1-227 truncation. |
| 260837 | pGEX-6P1-SPRTN-MPD (26-150) | Expression in E. coli of SPRTN-MPD domain (26-150). |
| 260838 | pGEX-6P1-SPRTN-MPD (26-150), L99A | Expression in E. coli of SPRTN-MPD domain (26-150), carrying the L99A mutation. |