DNA-induced conformational changes in SPRTN relieve its auto-inhibitory effect on protease activity.
Song W, Newman JA, Zhao Y, Chalk R, Redfield C, Elliott PR, Ramadan K
bioRxiv [Preprint]. 2026 May 1:2026.04.29.721615. doi: 10.64898/2026.04.29.721615.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260827 | pET-24-hexaUb | Expression in E. coli of linear hexa-ubiquitin. |
| 260828 | pET-24-octaUb | Expression in E. coli of linear octa-ubiquitin. |
| 260829 | pNIC28-SPRTN-MIU-SprT (1-214) | Expression in E. coli of SPRTN-MIU-SprT domain (1-214). |
| 260830 | pNIC28-SPRTN (1-227) | Expression in E. coli of SPRTN 1-227 truncation. |
| 260831 | pNIC28-SPRTN (26-240) (SprT-BR; SPRTN core), E112Amber | Expression in E. coli of SPRTN 26-240 truncation, carrying the E112Amber mutation. |
| 260832 | pNIC28-SPRTN-SprT (26-214) | Expression in E. coli of SPRTN 26-214 truncation. |
| 260833 | pNIC28-SPRTN-SprT (26-214), E112Amber | Expression in E. coli of SPRTN 26-214 truncation, carrying the E112Amber mutation. |
| 260834 | pNIC28-SPRTN-BR-SprT (BR-26-214) | Expression in E. coli of SPRTN 26-214 fused with BR on the N-terminus. |
| 260835 | pNIC28-SPRTN-BR-SprT (BR-26-214), E112Amber | Expression in E. coli of SPRTN 26-214 fused with BR on the N-terminus, carrying the E112Amber mutation. |
| 260836 | pGEX-6P1-SPRTN-ZBD (151-215) | Expression in E. coli of SPRTN-ZBD domain (151-215). |
| 260837 | pGEX-6P1-SPRTN-MPD (26-150) | Expression in E. coli of SPRTN-MPD domain (26-150). |
| 260838 | pGEX-6P1-SPRTN-MPD (26-150), L99A | Expression in E. coli of SPRTN-MPD domain (26-150), carrying the L99A mutation. |