PD2529 human β8 full-P2A-GFP
(Plasmid
#260943)
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PurposePlasmid encoding human integrin β8 full-length
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepD2529 CAG
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintegrin beta-8
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SpeciesH. sapiens (human)
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Entrez GeneITGB8
- Promoter CAG
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Tags
/ Fusion Proteins
- CD33 secretion peptide (N terminal on insert)
- P2A-AcGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit 10.1101/2024.01.26.577394 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PD2529 human β8 full-P2A-GFP was a gift from Timothy Springer (Addgene plasmid # 260943 ; http://n2t.net/addgene:260943 ; RRID:Addgene_260943) -
For your References section:
Synthetic integrin antibodies discovered by yeast display reveal alphaV subunit pairing preferences with beta subunits. Hao Y, Yan J, Fraser C, Jiang A, Anuganti M, Zhang R, Lloyd K, Jardine J, Coppola J, Meijers R, Li J, Springer TA. MAbs. 2024 Jan-Dec;16(1):2365891. doi: 10.1080/19420862.2024.2365891. Epub 2024 Jun 18. 10.1080/19420862.2024.2365891 PubMed 38889315