pUG62_Lvl0_P2_ NatR-FRTwt
(Plasmid
#261037)
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PurposeLevel 0 part. Nourseothricin resistance cassette with FRT sites for flippase-mediated recombination.
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Depositing Lab
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Depositing OrganizationHeinrich-Heine-Universitaet Duesseldorf
Located in Germany -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 261037 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepICH41308
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Backbone manufacturerSylvestre Marillonnet (Addgene #47998)
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNatR-FRTwt
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SpeciesS. cerevisiae (budding yeast); Streptomyces noursei, Ustilago maydis
- Promoter Ptdh3short
Cloning Information
- Cloning method Golden Gate
Resource Information
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A portion of this plasmid was derived from a plasmid made byTerfruchte et al. 2014, doi.org/10.1016/j.fgb.2013.10.012
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: pUX475. Please visit https://doi.org/10.64898/2026.06.11.731564 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUG62_Lvl0_P2_ NatR-FRTwt was a gift from Kerstin Schipper (Addgene plasmid # 261037 ; http://n2t.net/addgene:261037 ; RRID:Addgene_261037)
